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Daniel

Oral and Biomedical Sciences PhD student at Cardiff University. Interested in Candida, oral biofilms, microbial interactions, host cell responses to infections and tissue engineering. Father to two amazing boys, and husband to an incredible wife. Hobbies include writing, listening to and performing music with my band and spending time with the family.

Maths is not my strong point

Today, after being here at Cardiff University for about three and a half months doing my PhD project..working with bacteria, enumerating etc which if I’m honest is not the easiest thing to do in your head quickly, and using log values and the such..

Today is not like any other day, today I received my calculator. Woo! Now, this may not seem like such a big deal, but boy does it make me happy!

And what’s the first thing you do when you get a new calculator…calculate of course! Had to be done 🙂

2014-01-23 11.15.01

 

DM

Catching up!

Wow, over three months since my last post..sorry about that!

So what have I been up to in the past three months?
– I’ve gotten stuck in with lab work; making acrylic pieces, doing adherence assays and growing biofilms on them (and looking at them using fluorescence confocal microscopy to determine depth and arrangement!). Also have done DNA extraction, run PCRs and purified samples (ready for sequencing) of my stock cultures.. all good fun but very important work for when my clinical samples start coming in!..
– Talking of clinical samples, IRAS (ethics) forms have been submitted and we are ready for the panel meeting which is going to be the start of Feb.
– Literature reading..and lots of it!
– Cardiff University have a yearly review for each PhD student, but instead of being at the end of the year (as it is called an End of Year Progress Report), its requested at 8 months!! This is a substantial report (9,000 words), including a significant literature review and details of my methods, results and conclusions to date.. all the hard lab work will be put on trial!

Since my last post, we’ve had Christmas and New Year – so happy new year to everyone that I haven’t yet seen/spoken to :). Christmas was lovely – spent a lot of time with the family which was great, Ruben had a blast with all his presents and it was really nice to be able to spend as much time with him as I was able to!

They say doing a PhD is 90% perseverance, 10% intelligence..and that is most definitely the case!! The trouble with growing biofilms, which grow for 7/14 days or more, is that if you get a single contaminant..that’s it! I found out the hard way.. one week in, I noticed that the media was changing colour by the end of each day and wondered why that was but just thought it was significant growth..no.. turns out I had contamination and all sorts grew when I enumerated my experiment! Three weeks worth of work (as I staggered the experiments) down the drain..just before Christmas!!! These experiments start up again this week, so fingers toes and everything that will physically cross are crossed in hope that this works (and I’ve stepped up my aseptic technique to avoid any issues 🙂 ).

One of my goals when starting the PhD was to do demonstrating/teaching at the undergraduate level. Well, my luck is in! I’ve managed to take part in a first year undergraduate medical students demonstrating scheme. I get a collection of 12 undergrads, and get them to do some practical work with me which is part of their curriculum (and it gives me teaching experience too!). I’ve chosen an adherence assay with some variables that are of interest to me, so they may even contribute toward my work progress in some way. I have to give them a presentation in March sometime, then they join me in May for a morning (in three groups of four students). Then they go away and analyse the results, and prepare a presentation of their work. They do a lot of the work, but I’m there to help point them in the right direction..should be good fun!

I think that’s enough rambling for now..I now have a reminder to post more frequently! 🙂

Ooh, and I got my first tattoo today!! #TattooTimes

Tattoo picture: 'If I have seen further, it is by standing on the shoulders of giants"
‘If I have seen further, it is by standing on the shoulders of giants”

DM

First week complete!

So my first week as a PhD student is complete – inductions complete, h&s stuff sorted, introductions to my academic colleagues done and settling in well.

Everybody is incredibly supportive; Rachael the amazing post-doc mum-like person, Kirsty my student buddy, Ray, Fraser, Niels, Lucy, Steve and fellow first year PhD student Paul!

Its like gaining a new family and an absolute pleasure to be a part of :). In at the mid-end next week (not quite the deep end..) with some PCR work on samples I sub cultured in my first week, ordered primers with Melanie which should arrive soon so that will be nice to do. Training day on Monday, and every monday for the foreseeable future!! Then just a lot more reading to do to try and get up to speed with the area. Fun!

Heres a picture of my new home 🙂

Cardiff uni desk
New home

Getting stuck in (kinda)

So, my first week as a PhD student has started. Generally quite organised – inductions were good and informative, and actually not boring! Lots of reading to be done, and just learning my way around is the biggest challenge at the moment!

Everybody is really friendly – the guys in the office are great..very supportive indeed!

Going to get stuck in tomorrow with my second supervisor..working with Candida isolates – subculturing just to get my feet wet, and then more literature reading and background searching! Its not boring yet..but that has the potential for change too!!

Nearly there

So, had a great meeting with my PI on Friday. Discussed ethics information for submission to the uni, contacts and provided some signatures, so its all systems go!

Now I have my university login details, time to start reading, reviewing, noting and learning about my project area..which means obtaining and spending some important time reading journals, papers etc. You know what it’s like if you have ever completed a dissertation project, or any kind of research essay! Cumbersome, mentally exhausting but so important, its not worth not doing!

Back to it! I’ll let you know how it goes 😀 just four weeks to go ’til I ‘start’!

And so it begins…

Hi, and welcome to my blog 🙂

I am an R&D Scientist; but not for much longer. In October of this year I embark on a PhD studentship at Cardiff University – School of Dentistry to undertake a research PhD investigating biofilms in denture stomatitis. It is a very exciting chapter in my life, and one that will inevitably make me an all round better person; personally and professionally.

It goes without saying that this next few years is going to be the most difficult of my life so far, but already feels as though it is going to be the most exciting (apart from the birth of my son or my wedding day..of course!). I am very much looking forward to the new challenges that lie ahead and with the support of my family know that it will be a huge success.

This blog is really just an ongoing diary of my studentship and goings-on in my life, so come and join me in my journey 🙂

Daniel