Highs and lows and highs

This week was filled with anticipation; ELISA kits due to arrive, antibodies due to arrive, tissue models ready to be used for infections.. However, it quickly became apparent that this week was going to be a tough one!

After chaining the medium of the tissue models (all 36 of them..) on Monday, all was well. Until Wednesday. During the medium change on Wednesday, I noticed the medium was cloudy, and upon a quick look under the microscope I found the wells packed with what look like fungal spores, or yeast cells (Candida?)! Such a shame as these had been growing so well for about 3 weeks, and only now, two days before they were needed, they were infected. I collected some and took them for pathological analysis anyway to check the structure, and this is where the good bits come into play – the structure seems pretty good for a first attempt at tissues (and with cells that are known to be poor at tissue models!). H&E staining was great, and the thickness looks pretty good, just need to wait for a proper pathologist to take a look and critique!

Another high point was the arrival of the ELISA kit which now works properly! Thursday was a great day for this – the protocol finished, and showed a brilliant set of results – nearly exactly what I wanted to see – LPS influences pro-inflammatory cytokine release of monocyte cells :D. More work to be done..but watch this space!

And.. after 2 1/2 years here, I had my first fire alarm experience…and then another today! Two in two days, but nothing for the whole time I was here before. Thankfully no fire or damage phew, and all is well!

Busy week ahead with more monocyte priming/challenge work to be done, flow work, ELISA, qPCR and biofilms!! Busy busy 🙂
DM

Daniel

Postdoctoral Research Associate interested in Candida, oral biofilms, virulence, microbial interactions, host cell responses to infections and tissue engineering. Father to two amazing boys, and husband to an incredible wife. Hobbies include writing, listening to and performing music with my band and spending time with the family.

Leave a Reply

This site uses Akismet to reduce spam. Learn how your comment data is processed.